Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3944103..3944757 Replicon chromosome
Accession NZ_CP028732
Organism Escherichia coli strain H3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag AOY60_RS19115 Protein ID WP_000244777.1
Coordinates 3944103..3944510 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY60_RS19120 Protein ID WP_000354046.1
Coordinates 3944491..3944757 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY60_RS19095 (3940060) 3940060..3941793 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
AOY60_RS19100 (3941799) 3941799..3942509 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY60_RS19105 (3942534) 3942534..3943430 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY60_RS19110 (3943542) 3943542..3944063 + 522 WP_001055874.1 flavodoxin FldB -
AOY60_RS19115 (3944103) 3944103..3944510 - 408 WP_000244777.1 protein YgfX Toxin
AOY60_RS19120 (3944491) 3944491..3944757 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY60_RS19125 (3945000) 3945000..3945980 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
AOY60_RS19130 (3946176) 3946176..3946835 - 660 WP_000250274.1 hemolysin III family protein -
AOY60_RS19135 (3946999) 3946999..3947310 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
AOY60_RS19140 (3947355) 3947355..3948788 + 1434 WP_001573179.1 6-phospho-beta-glucosidase BglA -
AOY60_RS19145 (3948845) 3948845..3949588 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T103121 WP_000244777.1 NZ_CP028732:c3944510-3944103 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T103121 NZ_CP028732:c3944510-3944103 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT103121 WP_000354046.1 NZ_CP028732:c3944757-3944491 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT103121 NZ_CP028732:c3944757-3944491 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References