Detailed information of TA system TA103107

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1976893..1977686
• Replicon: chromosome
• Accession: NZ_CP028732
• Organism: Escherichia coli strain H3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: AOY60_RS09340
• Protein ID: WP_000854686.1
• Coordinates: 1977303..1977686 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: AOY60_RS09335
• Protein ID: WP_201473033.1
• Coordinates: 1976893..1977222 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY60_RS09320 (1973420)	1973420..1974325	+	906	WP_000203541.1	diguanylate cyclase regulator RdcB family protein	-
AOY60_RS09325 (1974322)	1974322..1975392	+	1071	WP_000102669.1	patatin-like phospholipase family protein	-
AOY60_RS09330 (1975732)	1975732..1976550	+	819	WP_001234685.1	DUF932 domain-containing protein	-
AOY60_RS09335 (1976893)	1976893..1977222	+	330	WP_201473033.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AOY60_RS09340 (1977303)	1977303..1977686	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
AOY60_RS09345 (1977683)	1977683..1978171	+	489	WP_001054233.1	DUF5983 family protein	-
AOY60_RS09350 (1978188)	1978188..1978385	+	198	WP_000839253.1	DUF957 domain-containing protein	-
AOY60_RS09355 (1978470)	1978470..1979312	+	843	WP_001431817.1	DUF4942 domain-containing protein	-
AOY60_RS09365 (1979783)	1979783..1980721	+	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
AOY60_RS09370 (1980776)	1980776..1981514	+	739	Protein_1820	zinc-binding phosphatase	-
AOY60_RS09375 (1981538)	1981538..1982092	+	555	WP_001001921.1	molecular chaperone YcdY	-
AOY60_RS09380 (1982194)	1982194..1982685	+	492	WP_001300785.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (20-100)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T103107 WP_000854686.1 NZ_CP028732:1977303-1977686 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T103107 NZ_CP028732:1977303-1977686 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12177.54 Da
• Isoelectric Point: 4.2662

>AT103107 WP_201473033.1 NZ_CP028732:1976893-1977222 [Escherichia coli]
MAPEPDDDDDETWVLFNAMNGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGL
TCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 330 bp

>AT103107 NZ_CP028732:1976893-1977222 [Escherichia coli]
ATGGCACCCGAACCGGATGACGATGATGACGAAACATGGGTACTGTTCAATGCCATGAACGGTAACCGGTTGCATTACCT
TGCAGACCGCGCCGGTATCAGAGGCCGGTTCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGA
AACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTG
ACCTGCGATGCCGACACCCTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCC
TCCGGAATAG