Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1287778..1288396 Replicon chromosome
Accession NZ_CP028732
Organism Escherichia coli strain H3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY60_RS06085 Protein ID WP_001291435.1
Coordinates 1287778..1287996 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY60_RS06090 Protein ID WP_000344800.1
Coordinates 1288022..1288396 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY60_RS06050 (1283067) 1283067..1283639 + 573 WP_000779838.1 YbaY family lipoprotein -
AOY60_RS06055 (1283670) 1283670..1283981 - 312 WP_000409911.1 MGMT family protein -
AOY60_RS06065 (1284360) 1284360..1284713 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY60_RS06070 (1284755) 1284755..1286305 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY60_RS06075 (1286469) 1286469..1286939 - 471 WP_000136192.1 YlaC family protein -
AOY60_RS06080 (1287055) 1287055..1287606 - 552 WP_000102564.1 maltose O-acetyltransferase -
AOY60_RS06085 (1287778) 1287778..1287996 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY60_RS06090 (1288022) 1288022..1288396 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY60_RS06095 (1288942) 1288942..1292091 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY60_RS06100 (1292114) 1292114..1293307 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T103106 WP_001291435.1 NZ_CP028732:c1287996-1287778 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T103106 NZ_CP028732:c1287996-1287778 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT103106 WP_000344800.1 NZ_CP028732:c1288396-1288022 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT103106 NZ_CP028732:c1288396-1288022 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References