103088

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 929531..930102 Replicon chromosome
Accession NZ_CP028727
Organism Enterococcus faecium strain FSIS1608820

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag C7I30_RS04805 Protein ID WP_002286801.1
Coordinates 929761..930102 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag C7I30_RS04800 Protein ID WP_002323011.1
Coordinates 929531..929761 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7I30_RS04755 924651..925982 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
C7I30_RS04760 926004..926630 + 627 WP_002293674.1 cysteine hydrolase -
C7I30_RS04765 926813..927394 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
C7I30_RS04785 928126..928701 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
C7I30_RS04790 928906..929244 - 339 WP_002286804.1 hypothetical protein -
C7I30_RS04800 929531..929761 + 231 WP_002323011.1 hypothetical protein Antitoxin
C7I30_RS04805 929761..930102 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C7I30_RS04810 930952..931137 + 186 WP_002304207.1 hypothetical protein -
C7I30_RS04815 931642..931836 + 195 WP_002295146.1 hypothetical protein -
C7I30_RS04820 932027..932276 + 250 Protein_850 transposase -
C7I30_RS04830 932520..933350 - 831 WP_002286789.1 manganese catalase family protein -
C7I30_RS04835 933558..934892 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T103088 WP_002286801.1 NZ_CP028727:929761-930102 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T103088 NZ_CP028727:929761-930102 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT103088 WP_002323011.1 NZ_CP028727:929531-929761 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT103088 NZ_CP028727:929531-929761 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References