103086

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 27487..28058 Replicon plasmid pN60443F-1
Accession NZ_CP028726
Organism Enterococcus faecalis strain CVM N60443F

Toxin (Protein)


Gene name mazF Uniprot ID A0A2P6BPI5
Locus tag C7I31_RS14160 Protein ID WP_002394791.1
Coordinates 27717..28058 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag C7I31_RS14155 Protein ID WP_002362431.1
Coordinates 27487..27717 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7I31_RS14120 22766..23803 + 1038 WP_002355369.1 PTS sugar transporter subunit IIC -
C7I31_RS14125 24108..24620 - 513 Protein_26 IS256-like element ISEfm2 family transposase -
C7I31_RS14130 24697..25799 + 1103 WP_131639410.1 IS3 family transposase -
C7I31_RS14140 26154..26375 - 222 WP_104866333.1 hypothetical protein -
C7I31_RS14145 26362..26673 - 312 WP_033628686.1 hypothetical protein -
C7I31_RS14150 26663..27283 - 621 WP_161971889.1 recombinase family protein -
C7I31_RS14155 27487..27717 + 231 WP_002362431.1 hypothetical protein Antitoxin
C7I31_RS14160 27717..28058 + 342 WP_002394791.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C7I31_RS14165 28170..29108 + 939 WP_002394789.1 hypothetical protein -
C7I31_RS14170 29376..29978 + 603 WP_002367780.1 Fic family protein -
C7I31_RS14175 30212..30892 - 681 WP_071621256.1 IS6 family transposase -
C7I31_RS14180 31211..31909 - 699 WP_002397506.1 Crp/Fnr family transcriptional regulator -
C7I31_RS14185 31926..32477 - 552 WP_002397505.1 DNA starvation/stationary phase protection protein -
C7I31_RS14190 32481..32717 - 237 WP_002397503.1 cation transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) ugd / bsh 1..53338 53338
- inside IScluster/Tn - bsh 17181..35802 18621


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13248.49 Da        Isoelectric Point: 8.8595

>T103086 WP_002394791.1 NZ_CP028726:27717-28058 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T103086 NZ_CP028726:27717-28058 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGTTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT103086 WP_002362431.1 NZ_CP028726:27487-27717 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp

>AT103086 NZ_CP028726:27487-27717 [Enterococcus faecalis]
ATGTTAACAACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P6BPI5

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References