103065

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 9328..9862 Replicon plasmid pN48037F-2
Accession NZ_CP028722
Organism Enterococcus faecalis strain CVM N48037F

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag C7I32_RS15650 Protein ID WP_002360769.1
Coordinates 9587..9862 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag C7I32_RS15645 Protein ID WP_002369771.1
Coordinates 9328..9594 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7I32_RS15615 5432..6217 + 786 WP_000803546.1 replication-relaxation family protein -
C7I32_RS15620 6223..6711 + 489 WP_002368088.1 hypothetical protein -
C7I32_RS15625 7303..7569 + 267 WP_025187532.1 hypothetical protein -
C7I32_RS15630 7602..8096 + 495 WP_002385540.1 DnaJ domain-containing protein -
C7I32_RS15635 8193..8609 + 417 WP_010830229.1 single-stranded DNA-binding protein -
C7I32_RS15640 8633..9217 + 585 WP_010830230.1 thermonuclease family protein -
C7I32_RS15645 9328..9594 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
C7I32_RS15650 9587..9862 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
C7I32_RS15660 10664..10849 + 186 WP_002368099.1 hypothetical protein -
C7I32_RS15665 10834..11025 + 192 WP_002385537.1 DpnD/PcfM family protein -
C7I32_RS15670 11245..12024 + 780 WP_002385536.1 hypothetical protein -
C7I32_RS15675 12024..13367 + 1344 WP_048942587.1 PcfJ domain-containing protein -
C7I32_RS15685 14058..14786 + 729 WP_025187535.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - prgB/asc10 1..50811 50811


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T103065 WP_002360769.1 NZ_CP028722:9587-9862 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T103065 NZ_CP028722:9587-9862 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT103065 WP_002369771.1 NZ_CP028722:9328-9594 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT103065 NZ_CP028722:9328-9594 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References