Detailed information of TA system TA103064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1485895..1486922
• Replicon: chromosome
• Accession: NZ_CP028720
• Organism: Enterococcus faecalis strain CVM N48037F

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: C7I32_RS07640
• Protein ID: WP_016633978.1
• Coordinates: 1485895..1486548 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: C7I32_RS07645
• Protein ID: WP_010816769.1
• Coordinates: 1486545..1486922 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7I32_RS07610	1481534..1481863	-	330	WP_010816770.1	helix-turn-helix transcriptional regulator	-
C7I32_RS07615	1482085..1482666	+	582	WP_033594342.1	phosphoglycerate mutase family protein	-
C7I32_RS07620	1482683..1483102	+	420	WP_002365234.1	MerR family transcriptional regulator	-
C7I32_RS07625	1483178..1484053	+	876	WP_033594341.1	aldo/keto reductase	-
C7I32_RS07630	1484153..1485484	+	1332	WP_016633980.1	FAD-dependent oxidoreductase	-
C7I32_RS07635	1485522..1485857	-	336	WP_033592588.1	helix-turn-helix transcriptional regulator	-
C7I32_RS07640	1485895..1486548	-	654	WP_016633978.1	ImmA/IrrE family metallo-endopeptidase	Toxin
C7I32_RS07645	1486545..1486922	-	378	WP_010816769.1	helix-turn-helix transcriptional regulator	Antitoxin
C7I32_RS07650	1487658..1487909	+	252	WP_002408313.1	hypothetical protein	-
C7I32_RS07655	1487995..1488321	+	327	WP_033594340.1	hypothetical protein	-
C7I32_RS07660	1488321..1488737	+	417	WP_033594339.1	DUF961 family protein	-
C7I32_RS16185	1488760..1488921	+	162	WP_002408316.1	hypothetical protein	-
C7I32_RS07665	1488908..1489096	-	189	WP_002371434.1	hypothetical protein	-
C7I32_RS07670	1489328..1489552	+	225	WP_010824177.1	hypothetical protein	-
C7I32_RS16190	1489579..1489731	+	153	WP_002399525.1	hypothetical protein	-
C7I32_RS07680	1489742..1489981	+	240	WP_033594338.1	hypothetical protein	-
C7I32_RS07685	1490021..1490344	+	324	WP_002399121.1	hypothetical protein	-
C7I32_RS07690	1490404..1490736	+	333	WP_131501885.1	nucleotidyltransferase domain-containing protein	-
C7I32_RS07695	1490720..1491088	+	369	WP_002365216.1	DUF86 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1468752..1549932	81180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (80-181)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-61)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 26064.97 Da
• Isoelectric Point: 5.9553

>T103064 WP_016633978.1 NZ_CP028720:c1486548-1485895 [Enterococcus faecalis]
MIGLLLVDFISKELYFEAFNFSNELIKEVSYYSSKNIEQVKCFDIEKYVKEVEDVEFVEYTFQKQLKRRMLGSISKVDDE
VIITTNKELMLERKNFTKMHEIMHYYIDIPKINNATHTFSDILLKNGYLMEDFPKEYRANVGASMLMANDQALFYALKNF
YSFEEIAQYFFMSKSALRNRLIEHLMYVNNCTFAHANTLFNNYYFHKETDIYRFIFN

Nucleotide

• Length: 654 bp

>T103064 NZ_CP028720:c1486548-1485895 [Enterococcus faecalis]
TTGATTGGACTGTTGTTGGTGGATTTTATTAGTAAAGAACTCTATTTTGAAGCATTTAATTTTTCGAACGAATTAATAAA
AGAAGTATCTTACTACTCTTCCAAAAATATTGAACAAGTTAAATGCTTTGATATTGAAAAATATGTAAAAGAAGTTGAAG
ATGTTGAATTTGTTGAATACACGTTTCAAAAACAACTTAAAAGACGGATGCTTGGTTCAATCTCTAAAGTAGACGATGAA
GTCATTATAACAACAAACAAGGAATTAATGTTAGAACGAAAAAATTTCACTAAAATGCATGAAATTATGCATTACTACAT
AGATATACCTAAAATTAATAATGCAACCCATACTTTTTCTGATATTCTTTTGAAAAATGGTTATTTGATGGAAGATTTTC
CAAAAGAATATCGTGCTAATGTCGGTGCTAGCATGTTAATGGCAAATGACCAAGCCTTGTTTTACGCATTAAAAAATTTT
TATTCATTCGAAGAAATTGCACAATACTTTTTTATGAGCAAATCTGCATTAAGAAATAGACTCATTGAACATCTTATGTA
TGTAAATAACTGTACTTTTGCACACGCTAATACACTTTTTAACAATTATTACTTTCATAAAGAAACTGATATCTACAGAT
TTATTTTCAATTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 14679.61 Da
• Isoelectric Point: 4.7690

>AT103064 WP_010816769.1 NZ_CP028720:c1486922-1486545 [Enterococcus faecalis]
METYKKIKSLRENKNVTQQEIADAINMNISVYKKIELGSRPIRENELSKIADFFNVSSDFLLNRTSNPLLPKDEDSIGQN
IISHFRLNTSDMDIEDIEELEEELIDFQDFLIKKAKEKKERNKKD

Nucleotide

• Length: 378 bp

>AT103064 NZ_CP028720:c1486922-1486545 [Enterococcus faecalis]
ATGGAAACTTACAAAAAAATTAAATCACTAAGAGAAAACAAAAATGTTACTCAACAAGAAATAGCCGATGCAATCAATAT
GAATATAAGTGTCTATAAAAAAATTGAATTAGGATCTCGCCCGATAAGAGAAAATGAACTTTCAAAAATCGCAGACTTCT
TCAACGTATCTTCAGATTTTCTTTTAAATAGAACTTCAAATCCTTTACTACCTAAAGATGAAGATAGCATCGGACAAAAT
ATTATTTCTCATTTTCGTTTAAATACTTCTGATATGGACATAGAAGATATAGAAGAATTAGAAGAGGAATTAATCGACTT
TCAAGATTTTCTTATAAAAAAAGCAAAAGAAAAAAAGGAACGTAACAAAAAAGATTGA