Detailed information of TA system TA103046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1155967..1156538
• Replicon: chromosome
• Accession: NZ_CP028720
• Organism: Enterococcus faecalis strain CVM N48037F

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C7I32_RS06055
• Protein ID: WP_002354774.1
• Coordinates: 1156197..1156538 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: C7I32_RS06050
• Protein ID: WP_002354773.1
• Coordinates: 1155967..1156197 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7I32_RS06030	1152444..1153739	+	1296	WP_033626639.1	AAA family ATPase	-
C7I32_RS06035	1153748..1154380	+	633	WP_002358972.1	RloB family protein	-
C7I32_RS06040	1154447..1155439	-	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
C7I32_RS06045	1155578..1155793	+	216	WP_002354768.1	zinc ribbon domain-containing protein	-
C7I32_RS06050	1155967..1156197	+	231	WP_002354773.1	hypothetical protein	Antitoxin
C7I32_RS06055	1156197..1156538	+	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C7I32_RS06065	1156909..1160523	+	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T103046 WP_002354774.1 NZ_CP028720:1156197-1156538 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T103046 NZ_CP028720:1156197-1156538 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT103046 WP_002354773.1 NZ_CP028720:1155967-1156197 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT103046 NZ_CP028720:1155967-1156197 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812