Detailed information of TA system TA102981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 696434..697029
• Replicon: chromosome
• Accession: NZ_CP028703
• Organism: Escherichia coli strain ME8067

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: DBB47_RS03580
• Protein ID: WP_108118561.1
• Coordinates: 696679..697029 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: DBB47_RS03575
• Protein ID: WP_001223208.1
• Coordinates: 696434..696685 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBB47_RS03565	692099..695878	+	3780	WP_000060911.1	autotransporter assembly complex protein TamB	-
DBB47_RS03570	695881..696222	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
DBB47_RS03575	696434..696685	+	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
DBB47_RS03580	696679..697029	+	351	WP_108118561.1	endoribonuclease toxin ChpB	Toxin
DBB47_RS03585	697109..697639	-	531	WP_000055075.1	inorganic diphosphatase	-
DBB47_RS03590	697949..698905	+	957	WP_000265913.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
DBB47_RS03595	699045..700547	+	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
DBB47_RS03600	700561..701583	+	1023	WP_001313531.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12507.37 Da
• Isoelectric Point: 5.5572

>T102981 WP_108118561.1 NZ_CP028703:696679-697029 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQQGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T102981 NZ_CP028703:696679-697029 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACAGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT102981 WP_001223208.1 NZ_CP028703:696434-696685 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT102981 NZ_CP028703:696434-696685 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26