Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3345862..3346445 Replicon chromosome
Accession NZ_CP028702
Organism Escherichia coli strain J53

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag DBB46_RS17460 Protein ID WP_000254738.1
Coordinates 3345862..3346197 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag DBB46_RS17465 Protein ID WP_000581937.1
Coordinates 3346197..3346445 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB46_RS17445 3341749..3343047 - 1299 WP_000036723.1 phosphopyruvate hydratase -
DBB46_RS17450 3343135..3344772 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
DBB46_RS17455 3345000..3345791 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
DBB46_RS17460 3345862..3346197 - 336 WP_000254738.1 endoribonuclease MazF Toxin
DBB46_RS17465 3346197..3346445 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
DBB46_RS17470 3346523..3348757 - 2235 WP_000226815.1 GTP pyrophosphokinase -
DBB46_RS17475 3348805..3350106 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T102971 WP_000254738.1 NZ_CP028702:c3346197-3345862 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T102971 NZ_CP028702:c3346197-3345862 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT102971 WP_000581937.1 NZ_CP028702:c3346445-3346197 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT102971 NZ_CP028702:c3346445-3346197 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References