Detailed information of TA system TA102967

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2509664..2510495
• Replicon: chromosome
• Accession: NZ_CP028702
• Organism: Escherichia coli strain J53

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: DBB46_RS13290
• Protein ID: WP_000854814.1
• Coordinates: 2510121..2510495 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: DBB46_RS13285
• Protein ID: WP_001285584.1
• Coordinates: 2509664..2510032 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBB46_RS13270	2507331..2508863	+	1533	WP_001350525.1	hypothetical protein	-
DBB46_RS13275	2508860..2509306	+	447	WP_000187523.1	RadC family protein	-
DBB46_RS13280	2509369..2509590	+	222	WP_000692323.1	DUF987 domain-containing protein	-
DBB46_RS13285	2509664..2510032	+	369	WP_001285584.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
DBB46_RS13290	2510121..2510495	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
DBB46_RS13295	2510492..2510686	+	195	WP_000988600.1	hypothetical protein	-
DBB46_RS24585	2510699..2510812	+	114	WP_001161660.1	DUF957 domain-containing protein	-
DBB46_RS13305	2511101..2511229	-	129	Protein_2429	transposase domain-containing protein	-
DBB46_RS13310	2511301..2511483	+	183	WP_001016348.1	hypothetical protein	-
DBB46_RS13315	2511584..2511913	-	330	WP_000450409.1	DUF496 family protein	-
DBB46_RS13320	2512085..2513143	-	1059	WP_001200891.1	FUSC family protein	-
DBB46_RS13325	2513341..2513814	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
DBB46_RS13330	2513933..2515099	-	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T102967 WP_000854814.1 NZ_CP028702:2510121-2510495 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T102967 NZ_CP028702:2510121-2510495 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT102967 WP_001285584.1 NZ_CP028702:2509664-2510032 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT102967 NZ_CP028702:2509664-2510032 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A1M2E8G6