Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2509664..2510495 Replicon chromosome
Accession NZ_CP028702
Organism Escherichia coli strain J53

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag DBB46_RS13290 Protein ID WP_000854814.1
Coordinates 2510121..2510495 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag DBB46_RS13285 Protein ID WP_001285584.1
Coordinates 2509664..2510032 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB46_RS13270 2507331..2508863 + 1533 WP_001350525.1 hypothetical protein -
DBB46_RS13275 2508860..2509306 + 447 WP_000187523.1 RadC family protein -
DBB46_RS13280 2509369..2509590 + 222 WP_000692323.1 DUF987 domain-containing protein -
DBB46_RS13285 2509664..2510032 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
DBB46_RS13290 2510121..2510495 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
DBB46_RS13295 2510492..2510686 + 195 WP_000988600.1 hypothetical protein -
DBB46_RS24585 2510699..2510812 + 114 WP_001161660.1 DUF957 domain-containing protein -
DBB46_RS13305 2511101..2511229 - 129 Protein_2429 transposase domain-containing protein -
DBB46_RS13310 2511301..2511483 + 183 WP_001016348.1 hypothetical protein -
DBB46_RS13315 2511584..2511913 - 330 WP_000450409.1 DUF496 family protein -
DBB46_RS13320 2512085..2513143 - 1059 WP_001200891.1 FUSC family protein -
DBB46_RS13325 2513341..2513814 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
DBB46_RS13330 2513933..2515099 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T102967 WP_000854814.1 NZ_CP028702:2510121-2510495 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T102967 NZ_CP028702:2510121-2510495 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT102967 WP_001285584.1 NZ_CP028702:2509664-2510032 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT102967 NZ_CP028702:2509664-2510032 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References