Detailed information of TA system TA102956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1942614..1943252
• Replicon: chromosome
• Accession: NZ_CP028702
• Organism: Escherichia coli strain J53

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: DBB46_RS10210
• Protein ID: WP_000813794.1
• Coordinates: 1942614..1942790 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DBB46_RS10215
• Protein ID: WP_001270286.1
• Coordinates: 1942836..1943252 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBB46_RS10190	1938233..1939447	-	1215	WP_001326689.1	BenE family transporter YdcO	-
DBB46_RS10195	1939500..1940036	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
DBB46_RS10200	1940109..1942070	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
DBB46_RS10205	1942162..1942392	-	231	WP_000494244.1	YncJ family protein	-
DBB46_RS10210	1942614..1942790	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
DBB46_RS10215	1942836..1943252	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
DBB46_RS10220	1943331..1944737	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
DBB46_RS10225	1944982..1946127	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
DBB46_RS10230	1946145..1947158	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
DBB46_RS10235	1947159..1948100	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T102956 WP_000813794.1 NZ_CP028702:1942614-1942790 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T102956 NZ_CP028702:1942614-1942790 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT102956 WP_001270286.1 NZ_CP028702:1942836-1943252 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT102956 NZ_CP028702:1942836-1943252 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB