Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48862..49387 Replicon plasmid pTA108
Accession NZ_CP028694
Organism Escherichia coli strain 108

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag C9E37_RS28080 Protein ID WP_001159868.1
Coordinates 49082..49387 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C9E37_RS28075 Protein ID WP_000813634.1
Coordinates 48862..49080 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E37_RS28040 44146..44376 + 231 WP_010891288.1 hypothetical protein -
C9E37_RS28045 44497..45237 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
C9E37_RS28050 45522..46498 - 977 Protein_42 RepB family plasmid replication initiator protein -
C9E37_RS28060 46906..47106 - 201 WP_000708307.1 hypothetical protein -
C9E37_RS28065 47103..47723 - 621 WP_001248529.1 hypothetical protein -
C9E37_RS28070 47720..48403 - 684 WP_010891291.1 DNA-binding protein -
C9E37_RS28075 48862..49080 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C9E37_RS28080 49082..49387 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
C9E37_RS28085 49388..50194 + 807 WP_000016989.1 site-specific integrase -
C9E37_RS28095 50917..52129 - 1213 Protein_49 IS3 family transposase -
C9E37_RS28100 52091..52429 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
C9E37_RS28105 53017..54183 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92730 92730


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T102838 WP_001159868.1 NZ_CP028694:49082-49387 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T102838 NZ_CP028694:49082-49387 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT102838 WP_000813634.1 NZ_CP028694:48862-49080 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT102838 NZ_CP028694:48862-49080 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References