Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2467464..2467935 Replicon chromosome
Accession NZ_CP028687
Organism Escherichia coli strain 110

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C9E39_RS12500 Protein ID WP_001303511.1
Coordinates 2467464..2467742 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C9E39_RS12505 Protein ID WP_001302048.1
Coordinates 2467744..2467935 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E39_RS12465 2462689..2462940 - 252 WP_000005552.1 excisionase family protein -
C9E39_RS12470 2463013..2465484 - 2472 WP_000048458.1 exonuclease -
C9E39_RS12475 2465577..2465768 - 192 WP_001090200.1 DUF1482 family protein -
C9E39_RS12480 2465765..2465953 - 189 WP_000449192.1 cell division inhibition protein DicB -
C9E39_RS12490 2466522..2466740 - 219 WP_001171930.1 hypothetical protein -
C9E39_RS12495 2466812..2467111 - 300 WP_001240334.1 hypothetical protein -
C9E39_RS12500 2467464..2467742 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9E39_RS12505 2467744..2467935 - 192 WP_001302048.1 hypothetical protein Antitoxin
C9E39_RS12510 2467956..2468327 - 372 WP_001169686.1 hypothetical protein -
C9E39_RS12515 2468425..2468727 + 303 WP_000172738.1 transcriptional regulator -
C9E39_RS12520 2468724..2469149 + 426 WP_000693943.1 Rha family transcriptional regulator -
C9E39_RS12525 2469172..2470134 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
C9E39_RS12530 2470141..2470881 + 741 WP_000788938.1 ATP-binding protein -
C9E39_RS12535 2470907..2471676 + 770 Protein_2405 DUF1627 domain-containing protein -
C9E39_RS12540 2471692..2472087 + 396 WP_001118159.1 DUF977 family protein -
C9E39_RS12545 2472144..2472728 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleA/espI 2452584..2507258 54674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T102754 WP_001303511.1 NZ_CP028687:c2467742-2467464 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T102754 NZ_CP028687:c2467742-2467464 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT102754 WP_001302048.1 NZ_CP028687:c2467935-2467744 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT102754 NZ_CP028687:c2467935-2467744 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References