Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4221249..4221867 Replicon chromosome
Accession NZ_CP028685
Organism Escherichia coli strain 111

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C9E40_RS22150 Protein ID WP_001291435.1
Coordinates 4221649..4221867 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C9E40_RS22145 Protein ID WP_000344800.1
Coordinates 4221249..4221623 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E40_RS22135 4216338..4217531 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C9E40_RS22140 4217554..4220703 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
C9E40_RS22145 4221249..4221623 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C9E40_RS22150 4221649..4221867 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C9E40_RS22155 4222039..4222590 + 552 WP_000102553.1 maltose O-acetyltransferase -
C9E40_RS22160 4222706..4223176 + 471 WP_000136192.1 YlaC family protein -
C9E40_RS22165 4223340..4224890 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C9E40_RS22170 4224932..4225285 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C9E40_RS22180 4225664..4225975 + 312 WP_000409911.1 MGMT family protein -
C9E40_RS22185 4226008..4226580 - 573 WP_137107943.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T102728 WP_001291435.1 NZ_CP028685:4221649-4221867 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T102728 NZ_CP028685:4221649-4221867 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT102728 WP_000344800.1 NZ_CP028685:4221249-4221623 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT102728 NZ_CP028685:4221249-4221623 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References