Detailed information of TA system TA102585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2464489..2464960
• Replicon: chromosome
• Accession: NZ_CP028674
• Organism: Escherichia coli strain 115

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C9E44_RS12450
• Protein ID: WP_001303511.1
• Coordinates: 2464489..2464767 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C9E44_RS12455
• Protein ID: WP_001302048.1
• Coordinates: 2464769..2464960 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E44_RS12410	2459495..2459679	-	185	Protein_2379	DUF3596 domain-containing protein	-
C9E44_RS12415	2459714..2459965	-	252	WP_000005552.1	excisionase family protein	-
C9E44_RS12420	2460038..2462509	-	2472	WP_000048458.1	exonuclease	-
C9E44_RS12425	2462602..2462793	-	192	WP_001090200.1	DUF1482 family protein	-
C9E44_RS12430	2462790..2462978	-	189	WP_000449192.1	cell division inhibition protein DicB	-
C9E44_RS12440	2463547..2463765	-	219	WP_001171930.1	hypothetical protein	-
C9E44_RS12445	2463837..2464136	-	300	WP_001240334.1	hypothetical protein	-
C9E44_RS12450	2464489..2464767	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E44_RS12455	2464769..2464960	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C9E44_RS12460	2464981..2465352	-	372	WP_001169686.1	hypothetical protein	-
C9E44_RS12465	2465450..2465752	+	303	WP_000172738.1	transcriptional regulator	-
C9E44_RS12470	2465749..2466174	+	426	WP_000693943.1	Rha family transcriptional regulator	-
C9E44_RS12475	2466197..2467159	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
C9E44_RS12480	2467166..2467906	+	741	WP_000788936.1	ATP-binding protein	-
C9E44_RS12485	2467932..2468701	+	770	Protein_2393	DUF1627 domain-containing protein	-
C9E44_RS12490	2468717..2469112	+	396	WP_001118159.1	DUF977 family protein	-
C9E44_RS12495	2469169..2469753	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2449609..2565221	115612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T102585 WP_001303511.1 NZ_CP028674:c2464767-2464489 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T102585 NZ_CP028674:c2464767-2464489 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT102585 WP_001302048.1 NZ_CP028674:c2464960-2464769 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT102585 NZ_CP028674:c2464960-2464769 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0