Detailed information of TA system TA102549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2521896..2522367
• Replicon: chromosome
• Accession: NZ_CP028671
• Organism: Escherichia coli strain 116

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C9E45_RS12905
• Protein ID: WP_001303511.1
• Coordinates: 2521896..2522174 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C9E45_RS12910
• Protein ID: WP_001302048.1
• Coordinates: 2522176..2522367 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E45_RS12865	2516902..2517086	-	185	Protein_2459	DUF3596 domain-containing protein	-
C9E45_RS12870	2517121..2517372	-	252	WP_000005552.1	excisionase family protein	-
C9E45_RS12875	2517445..2519916	-	2472	WP_000048458.1	exonuclease	-
C9E45_RS12880	2520009..2520200	-	192	WP_001090200.1	DUF1482 family protein	-
C9E45_RS12885	2520197..2520385	-	189	WP_000449192.1	cell division inhibition protein DicB	-
C9E45_RS12895	2520954..2521172	-	219	WP_001171930.1	hypothetical protein	-
C9E45_RS12900	2521244..2521543	-	300	WP_001240334.1	hypothetical protein	-
C9E45_RS12905	2521896..2522174	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E45_RS12910	2522176..2522367	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C9E45_RS12915	2522388..2522759	-	372	WP_001169686.1	hypothetical protein	-
C9E45_RS12920	2522857..2523159	+	303	WP_000172738.1	transcriptional regulator	-
C9E45_RS12925	2523156..2523581	+	426	WP_000693943.1	Rha family transcriptional regulator	-
C9E45_RS12930	2523604..2524566	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
C9E45_RS12935	2524573..2525313	+	741	WP_000788936.1	ATP-binding protein	-
C9E45_RS12940	2525339..2526108	+	770	Protein_2473	DUF1627 domain-containing protein	-
C9E45_RS12945	2526124..2526519	+	396	WP_001118159.1	DUF977 family protein	-
C9E45_RS12950	2526576..2527160	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2507016..2629528	122512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T102549 WP_001303511.1 NZ_CP028671:c2522174-2521896 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T102549 NZ_CP028671:c2522174-2521896 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT102549 WP_001302048.1 NZ_CP028671:c2522367-2522176 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT102549 NZ_CP028671:c2522367-2522176 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0