Detailed information of TA system TA102513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2522034..2522505
• Replicon: chromosome
• Accession: NZ_CP028668
• Organism: Escherichia coli strain 117

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C9E46_RS12910
• Protein ID: WP_001303511.1
• Coordinates: 2522034..2522312 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C9E46_RS12915
• Protein ID: WP_001302048.1
• Coordinates: 2522314..2522505 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E46_RS12870	2517040..2517224	-	185	Protein_2461	DUF3596 domain-containing protein	-
C9E46_RS12875	2517259..2517510	-	252	WP_000005552.1	excisionase family protein	-
C9E46_RS12880	2517583..2520054	-	2472	WP_000048458.1	exonuclease	-
C9E46_RS12885	2520147..2520338	-	192	WP_001090200.1	DUF1482 family protein	-
C9E46_RS12890	2520335..2520523	-	189	WP_000449192.1	cell division inhibition protein DicB	-
C9E46_RS12900	2521092..2521310	-	219	WP_001171930.1	hypothetical protein	-
C9E46_RS12905	2521382..2521681	-	300	WP_001240334.1	hypothetical protein	-
C9E46_RS12910	2522034..2522312	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E46_RS12915	2522314..2522505	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C9E46_RS12920	2522526..2522897	-	372	WP_001169686.1	hypothetical protein	-
C9E46_RS12925	2522995..2523297	+	303	WP_000172738.1	transcriptional regulator	-
C9E46_RS12930	2523294..2523719	+	426	WP_000693943.1	Rha family transcriptional regulator	-
C9E46_RS12935	2523742..2524704	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
C9E46_RS12940	2524711..2525451	+	741	WP_000788936.1	ATP-binding protein	-
C9E46_RS12945	2525477..2526246	+	770	Protein_2475	DUF1627 domain-containing protein	-
C9E46_RS12950	2526262..2526657	+	396	WP_001118159.1	DUF977 family protein	-
C9E46_RS12955	2526714..2527298	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2507154..2624078	116924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T102513 WP_001303511.1 NZ_CP028668:c2522312-2522034 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T102513 NZ_CP028668:c2522312-2522034 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT102513 WP_001302048.1 NZ_CP028668:c2522505-2522314 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT102513 NZ_CP028668:c2522505-2522314 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0