Detailed information of TA system TA102477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2521884..2522355
• Replicon: chromosome
• Accession: NZ_CP028665
• Organism: Escherichia coli strain 118

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C9E47_RS12905
• Protein ID: WP_001303511.1
• Coordinates: 2521884..2522162 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C9E47_RS12910
• Protein ID: WP_001302048.1
• Coordinates: 2522164..2522355 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E47_RS12865	2516890..2517074	-	185	Protein_2459	DUF3596 domain-containing protein	-
C9E47_RS12870	2517109..2517360	-	252	WP_000005552.1	excisionase family protein	-
C9E47_RS12875	2517433..2519904	-	2472	WP_000048458.1	exonuclease	-
C9E47_RS12880	2519997..2520188	-	192	WP_001090200.1	DUF1482 family protein	-
C9E47_RS12885	2520185..2520373	-	189	WP_000449192.1	cell division inhibition protein DicB	-
C9E47_RS12895	2520942..2521160	-	219	WP_001171930.1	hypothetical protein	-
C9E47_RS12900	2521232..2521531	-	300	WP_001240334.1	hypothetical protein	-
C9E47_RS12905	2521884..2522162	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E47_RS12910	2522164..2522355	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C9E47_RS12915	2522376..2522747	-	372	WP_001169686.1	hypothetical protein	-
C9E47_RS12920	2522845..2523147	+	303	WP_000172738.1	transcriptional regulator	-
C9E47_RS12925	2523144..2523569	+	426	WP_000693943.1	Rha family transcriptional regulator	-
C9E47_RS12930	2523592..2524554	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
C9E47_RS12935	2524561..2525301	+	741	WP_000788936.1	ATP-binding protein	-
C9E47_RS12940	2525327..2526096	+	770	Protein_2473	DUF1627 domain-containing protein	-
C9E47_RS12945	2526112..2526507	+	396	WP_001118159.1	DUF977 family protein	-
C9E47_RS12950	2526564..2527148	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2507004..2629515	122511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T102477 WP_001303511.1 NZ_CP028665:c2522162-2521884 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T102477 NZ_CP028665:c2522162-2521884 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT102477 WP_001302048.1 NZ_CP028665:c2522355-2522164 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT102477 NZ_CP028665:c2522355-2522164 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0