Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2469703..2470174 Replicon chromosome
Accession NZ_CP028654
Organism Escherichia coli strain 122

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C9E51_RS12515 Protein ID WP_001303511.1
Coordinates 2469703..2469981 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C9E51_RS12520 Protein ID WP_001302048.1
Coordinates 2469983..2470174 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E51_RS12480 2464928..2465179 - 252 WP_000005552.1 excisionase family protein -
C9E51_RS12485 2465252..2467723 - 2472 WP_137054076.1 exonuclease -
C9E51_RS12490 2467816..2468007 - 192 WP_001090200.1 DUF1482 family protein -
C9E51_RS12495 2468004..2468192 - 189 WP_000449192.1 cell division inhibition protein DicB -
C9E51_RS12505 2468761..2468979 - 219 WP_001171930.1 hypothetical protein -
C9E51_RS12510 2469051..2469350 - 300 WP_001240334.1 hypothetical protein -
C9E51_RS12515 2469703..2469981 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9E51_RS12520 2469983..2470174 - 192 WP_001302048.1 hypothetical protein Antitoxin
C9E51_RS12525 2470195..2470566 - 372 WP_001169686.1 hypothetical protein -
C9E51_RS12530 2470664..2470966 + 303 WP_000172738.1 transcriptional regulator -
C9E51_RS12535 2470963..2471388 + 426 WP_000693943.1 Rha family transcriptional regulator -
C9E51_RS12540 2471411..2472373 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
C9E51_RS12545 2472380..2473120 + 741 WP_000788938.1 ATP-binding protein -
C9E51_RS12550 2473146..2473915 + 770 Protein_2409 DUF1627 domain-containing protein -
C9E51_RS12555 2473931..2474326 + 396 WP_001118159.1 DUF977 family protein -
C9E51_RS12560 2474383..2474967 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2462542..2508156 45614


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T102337 WP_001303511.1 NZ_CP028654:c2469981-2469703 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T102337 NZ_CP028654:c2469981-2469703 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT102337 WP_001302048.1 NZ_CP028654:c2470174-2469983 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT102337 NZ_CP028654:c2470174-2469983 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References