Detailed information of TA system TA102236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2522003..2522474
• Replicon: chromosome
• Accession: NZ_CP028647
• Organism: Escherichia coli strain 130

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C9E54_RS12905
• Protein ID: WP_001303511.1
• Coordinates: 2522003..2522281 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C9E54_RS12910
• Protein ID: WP_001302048.1
• Coordinates: 2522283..2522474 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E54_RS12865	2517009..2517193	-	185	Protein_2459	DUF3596 domain-containing protein	-
C9E54_RS12870	2517228..2517479	-	252	WP_000005552.1	excisionase family protein	-
C9E54_RS12875	2517552..2520023	-	2472	WP_000048458.1	exonuclease	-
C9E54_RS12880	2520116..2520307	-	192	WP_001090200.1	DUF1482 family protein	-
C9E54_RS12885	2520304..2520492	-	189	WP_000449192.1	cell division inhibition protein DicB	-
C9E54_RS12895	2521061..2521279	-	219	WP_001171930.1	hypothetical protein	-
C9E54_RS12900	2521351..2521650	-	300	WP_001240334.1	hypothetical protein	-
C9E54_RS12905	2522003..2522281	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E54_RS12910	2522283..2522474	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C9E54_RS12915	2522495..2522866	-	372	WP_001169686.1	hypothetical protein	-
C9E54_RS12920	2522964..2523266	+	303	WP_000172738.1	transcriptional regulator	-
C9E54_RS12925	2523263..2523688	+	426	WP_000693943.1	Rha family transcriptional regulator	-
C9E54_RS12930	2523711..2524673	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
C9E54_RS12935	2524680..2525420	+	741	WP_000788936.1	ATP-binding protein	-
C9E54_RS12940	2525446..2526215	+	770	Protein_2473	DUF1627 domain-containing protein	-
C9E54_RS12945	2526231..2526626	+	396	WP_001118159.1	DUF977 family protein	-
C9E54_RS12950	2526683..2527267	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2501995..2629623	127628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T102236 WP_001303511.1 NZ_CP028647:c2522281-2522003 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T102236 NZ_CP028647:c2522281-2522003 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT102236 WP_001302048.1 NZ_CP028647:c2522474-2522283 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT102236 NZ_CP028647:c2522474-2522283 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0