Detailed information of TA system TA101993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3637263..3637968
• Replicon: chromosome
• Accession: NZ_CP028626
• Organism: Escherichia coli strain 137

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: C9E61_RS19035
• Protein ID: WP_000539519.1
• Coordinates: 3637263..3637649 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C9E61_RS19040
• Protein ID: WP_001280945.1
• Coordinates: 3637639..3637968 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E61_RS19015	3633267..3633893	+	627	WP_001295292.1	glutathione S-transferase GstB	-
C9E61_RS19020	3633890..3635005	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
C9E61_RS19025	3635116..3635499	-	384	WP_000497137.1	biofilm formation regulator BssR	-
C9E61_RS19030	3635712..3637037	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
C9E61_RS19035	3637263..3637649	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E61_RS19040	3637639..3637968	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
C9E61_RS19045	3638038..3639366	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
C9E61_RS19050	3639374..3641722	-	2349	WP_000950320.1	EAL domain-containing protein	-
C9E61_RS19055	3641900..3642811	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T101993 WP_000539519.1 NZ_CP028626:3637263-3637649 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T101993 NZ_CP028626:3637263-3637649 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT101993 WP_001280945.1 NZ_CP028626:3637639-3637968 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT101993 NZ_CP028626:3637639-3637968 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA