Detailed information of TA system TA101989

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3254889..3255684
• Replicon: chromosome
• Accession: NZ_CP028626
• Organism: Escherichia coli strain 137

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: C9E61_RS17020
• Protein ID: WP_000854914.1
• Coordinates: 3254889..3255263 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C9E61_RS17025
• Protein ID: WP_001416641.1
• Coordinates: 3255310..3255684 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E61_RS16980	3249893..3250384	-	492	WP_001301587.1	DUF1097 domain-containing protein	-
C9E61_RS16985	3250486..3251040	-	555	WP_001001917.1	molecular chaperone YcdY	-
C9E61_RS16990	3251064..3251801	-	738	WP_000283664.1	zinc-binding phosphatase	-
C9E61_RS16995	3251856..3252794	-	939	WP_000351284.1	glyoxylate/hydroxypyruvate reductase GhrA	-
C9E61_RS17005	3253265..3254107	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
C9E61_RS17010	3254192..3254389	-	198	WP_000839282.1	DUF957 domain-containing protein	-
C9E61_RS17015	3254401..3254892	-	492	WP_000976853.1	hypothetical protein	-
C9E61_RS17020	3254889..3255263	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
C9E61_RS17025	3255310..3255684	-	375	WP_001416641.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C9E61_RS17030	3255847..3256068	-	222	WP_001220314.1	DUF987 domain-containing protein	-
C9E61_RS17035	3256131..3256607	-	477	WP_001186738.1	RadC family protein	-
C9E61_RS17040	3256623..3257108	-	486	WP_000214398.1	antirestriction protein	-
C9E61_RS17045	3257199..3258017	-	819	WP_001234682.1	DUF945 domain-containing protein	-
C9E61_RS17055	3258357..3259427	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
C9E61_RS17060	3259424..3260329	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T101989 WP_000854914.1 NZ_CP028626:c3255263-3254889 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T101989 NZ_CP028626:c3255263-3254889 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13778.61 Da
• Isoelectric Point: 6.6249

>AT101989 WP_001416641.1 NZ_CP028626:c3255684-3255310 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGFCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT101989 NZ_CP028626:c3255684-3255310 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTTCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5