Detailed information of TA system TA101985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2522033..2522504
• Replicon: chromosome
• Accession: NZ_CP028626
• Organism: Escherichia coli strain 137

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C9E61_RS12910
• Protein ID: WP_001303511.1
• Coordinates: 2522033..2522311 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C9E61_RS12915
• Protein ID: WP_001302048.1
• Coordinates: 2522313..2522504 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E61_RS12870	2517039..2517223	-	185	Protein_2460	DUF3596 domain-containing protein	-
C9E61_RS12875	2517258..2517509	-	252	WP_000005552.1	excisionase family protein	-
C9E61_RS12880	2517582..2520053	-	2472	WP_000048458.1	exonuclease	-
C9E61_RS12885	2520146..2520337	-	192	WP_001090200.1	DUF1482 family protein	-
C9E61_RS12890	2520334..2520522	-	189	WP_000449192.1	cell division inhibition protein DicB	-
C9E61_RS12900	2521091..2521309	-	219	WP_001171930.1	hypothetical protein	-
C9E61_RS12905	2521381..2521680	-	300	WP_001240334.1	hypothetical protein	-
C9E61_RS12910	2522033..2522311	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E61_RS12915	2522313..2522504	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C9E61_RS12920	2522525..2522896	-	372	WP_001169686.1	hypothetical protein	-
C9E61_RS12925	2522994..2523296	+	303	WP_000172738.1	transcriptional regulator	-
C9E61_RS12930	2523293..2523718	+	426	WP_000693943.1	Rha family transcriptional regulator	-
C9E61_RS12935	2523741..2524703	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
C9E61_RS12940	2524710..2525450	+	741	WP_000788936.1	ATP-binding protein	-
C9E61_RS12945	2525476..2526245	+	770	Protein_2474	DUF1627 domain-containing protein	-
C9E61_RS12950	2526261..2526656	+	396	WP_001118159.1	DUF977 family protein	-
C9E61_RS12955	2526713..2527297	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2502025..2624075	122050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T101985 WP_001303511.1 NZ_CP028626:c2522311-2522033 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T101985 NZ_CP028626:c2522311-2522033 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT101985 WP_001302048.1 NZ_CP028626:c2522504-2522313 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT101985 NZ_CP028626:c2522504-2522313 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• AlphaFold DB: A0A829CTP0