Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 48863..49388 Replicon plasmid pTA138
Accession NZ_CP028625
Organism Escherichia coli strain 138

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag C9E62_RS28115 Protein ID WP_001159868.1
Coordinates 49083..49388 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C9E62_RS28110 Protein ID WP_000813634.1
Coordinates 48863..49081 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E62_RS28075 44146..44376 + 231 WP_010891288.1 hypothetical protein -
C9E62_RS28080 44497..45237 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
C9E62_RS28085 45522..46499 - 978 WP_001508846.1 RepB family plasmid replication initiator protein -
C9E62_RS28095 46907..47107 - 201 WP_000708307.1 hypothetical protein -
C9E62_RS28100 47104..47724 - 621 WP_001248529.1 hypothetical protein -
C9E62_RS28105 47721..48404 - 684 WP_010891291.1 DNA-binding protein -
C9E62_RS28110 48863..49081 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C9E62_RS28115 49083..49388 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
C9E62_RS28120 49389..50195 + 807 WP_000016989.1 site-specific integrase -
C9E62_RS28135 52207..52695 + 489 Protein_50 RepB family plasmid replication initiator protein -
C9E62_RS28140 52748..53961 + 1214 WP_162829202.1 IS3 family transposase -
C9E62_RS28145 54006..54275 + 270 WP_001241949.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..94493 94493


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T101967 WP_001159868.1 NZ_CP028625:49083-49388 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T101967 NZ_CP028625:49083-49388 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT101967 WP_000813634.1 NZ_CP028625:48863-49081 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT101967 NZ_CP028625:48863-49081 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References