Detailed information of TA system TA101895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 48857..49382
• Replicon: plasmid pTA140
• Accession: NZ_CP028619
• Organism: Escherichia coli strain 140

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: C9E64_RS28120
• Protein ID: WP_001159868.1
• Coordinates: 49077..49382 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: C9E64_RS28115
• Protein ID: WP_000813634.1
• Coordinates: 48857..49075 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E64_RS28080	44140..44370	+	231	WP_010891288.1	hypothetical protein	-
C9E64_RS28085	44491..45231	+	741	WP_001066920.1	tyrosine-type recombinase/integrase	-
C9E64_RS28090	45516..46493	-	978	WP_001508846.1	RepB family plasmid replication initiator protein	-
C9E64_RS28100	46901..47101	-	201	WP_000708307.1	hypothetical protein	-
C9E64_RS28105	47098..47718	-	621	WP_001248529.1	hypothetical protein	-
C9E64_RS28110	47715..48398	-	684	WP_010891291.1	DNA-binding protein	-
C9E64_RS28115	48857..49075	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
C9E64_RS28120	49077..49382	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
C9E64_RS28125	49383..50189	+	807	WP_000016989.1	site-specific integrase	-
C9E64_RS28140	52201..52689	+	489	Protein_50	RepB family plasmid replication initiator protein	-
C9E64_RS28145	52742..53955	+	1214	WP_162829202.1	IS3 family transposase	-
C9E64_RS28150	54000..54269	+	270	WP_001241949.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB	1..94487	94487

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T101895 WP_001159868.1 NZ_CP028619:49077-49382 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T101895 NZ_CP028619:49077-49382 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT101895 WP_000813634.1 NZ_CP028619:48857-49075 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT101895 NZ_CP028619:48857-49075 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2