Detailed information of TA system TA101841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2521668..2522139
• Replicon: chromosome
• Accession: NZ_CP028614
• Organism: Escherichia coli strain 141

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: C9E65_RS12905
• Protein ID: WP_001303511.1
• Coordinates: 2521668..2521946 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: C9E65_RS12910
• Protein ID: WP_001302048.1
• Coordinates: 2521948..2522139 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E65_RS12865	2516674..2516858	-	185	Protein_2459	DUF3596 domain-containing protein	-
C9E65_RS12870	2516893..2517144	-	252	WP_000005552.1	excisionase family protein	-
C9E65_RS12875	2517217..2519688	-	2472	WP_000048458.1	exonuclease	-
C9E65_RS12880	2519781..2519972	-	192	WP_001090200.1	DUF1482 family protein	-
C9E65_RS12885	2519969..2520157	-	189	WP_000449192.1	cell division inhibition protein DicB	-
C9E65_RS12895	2520726..2520944	-	219	WP_001171930.1	hypothetical protein	-
C9E65_RS12900	2521016..2521315	-	300	WP_001240334.1	hypothetical protein	-
C9E65_RS12905	2521668..2521946	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E65_RS12910	2521948..2522139	-	192	WP_001302048.1	hypothetical protein	Antitoxin
C9E65_RS12915	2522160..2522531	-	372	WP_001169686.1	hypothetical protein	-
C9E65_RS12920	2522629..2522931	+	303	WP_000172738.1	transcriptional regulator	-
C9E65_RS12925	2522928..2523353	+	426	WP_000693943.1	Rha family transcriptional regulator	-
C9E65_RS12930	2523376..2524338	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
C9E65_RS12935	2524345..2525085	+	741	WP_000788936.1	ATP-binding protein	-
C9E65_RS12940	2525111..2525880	+	770	Protein_2473	DUF1627 domain-containing protein	-
C9E65_RS12945	2525896..2526291	+	396	WP_001118159.1	DUF977 family protein	-
C9E65_RS12950	2526348..2526932	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2513381..2623713	110332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T101841 WP_001303511.1 NZ_CP028614:c2521946-2521668 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T101841 NZ_CP028614:c2521946-2521668 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT101841 WP_001302048.1 NZ_CP028614:c2522139-2521948 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT101841 NZ_CP028614:c2522139-2521948 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0