Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 2477..3036 Replicon plasmid pTA142-2
Accession NZ_CP028611
Organism Escherichia coli strain 142

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag C9E66_RS28130 Protein ID WP_000421257.1
Coordinates 2761..3036 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag C9E66_RS28125 Protein ID WP_001178089.1
Coordinates 2477..2761 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E66_RS28115 700..1572 + 873 WP_000729440.1 incFII family plasmid replication initiator RepA -
C9E66_RS28125 2477..2761 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
C9E66_RS28130 2761..3036 + 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9E66_RS28135 3142..3423 + 282 WP_001105066.1 hypothetical protein -
C9E66_RS28145 3614..3880 - 267 WP_000627831.1 hypothetical protein -
C9E66_RS28150 3880..4062 - 183 Protein_5 hypothetical protein -
C9E66_RS28155 4118..4720 - 603 WP_000517694.1 hypothetical protein -
C9E66_RS28165 5074..6420 + 1347 WP_001132021.1 hypothetical protein -
C9E66_RS29465 6919..7221 - 303 WP_001245884.1 hypothetical protein -
C9E66_RS28180 7218..7844 - 627 WP_000587689.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..88018 88018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T101821 WP_000421257.1 NZ_CP028611:2761-3036 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T101821 NZ_CP028611:2761-3036 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT101821 WP_001178089.1 NZ_CP028611:2477-2761 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT101821 NZ_CP028611:2477-2761 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References