Detailed information of TA system TA101816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1771..2318
• Replicon: plasmid pTA142-3
• Accession: NZ_CP028610
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C9E66_RS27610
• Protein ID: WP_000970004.1
• Coordinates: 2037..2318 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: W0FTF6
• Locus tag: C9E66_RS27605
• Protein ID: WP_000079942.1
• Coordinates: 1771..2040 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E66_RS27595	1..858	+	858	WP_000130991.1	incFII family plasmid replication initiator RepA	-
C9E66_RS27605	1771..2040	+	270	WP_000079942.1	hypothetical protein	Antitoxin
C9E66_RS27610	2037..2318	+	282	WP_000970004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E66_RS27615	2755..2991	+	237	WP_032257624.1	colicin V immunity protein	-
C9E66_RS27620	2969..3280	+	312	WP_001259758.1	colicin V	-
C9E66_RS27625	3450..5564	-	2115	WP_109996808.1	peptidase domain-containing ABC transporter	-
C9E66_RS27630	5539..6813	-	1275	WP_021573925.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67786	67786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10846.54 Da
• Isoelectric Point: 8.4615

>T101816 WP_000970004.1 NZ_CP028610:2037-2318 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLVAAIELDERIGYSASSLAGLPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T101816 NZ_CP028610:2037-2318 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGGT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCTGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10282.61 Da
• Isoelectric Point: 7.2057

>AT101816 WP_000079942.1 NZ_CP028610:1771-2040 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT101816 NZ_CP028610:1771-2040 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCAGCTACCGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A140JYS5