Detailed information of TA system TA010180

Experimentally validated

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/-
• Location: 1340982..1342359
• Replicon: chromosome
• Accession: NC_015680
• Organism: Pyrococcus yayanosii CH1

Toxin (Protein)

• Gene name: PygT
• Uniprot ID: F8AGR3
• Locus tag: PYCH_RS07505
• Protein ID: WP_013906255.1
• Coordinates: 1341598..1342359 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: PygA
• Uniprot ID: F8AGR2
• Locus tag: PYCH_RS07500
• Protein ID: WP_013906254.1
• Coordinates: 1340982..1341608 (+)
• Length: 209 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PYCH_RS07480 (PYCH_15280)	1337033..1338949	-	1917	WP_013906250.1	DUF87 domain-containing protein	-
PYCH_RS07485 (PYCH_15290)	1339130..1340020	-	891	WP_013906251.1	IS481 family transposase	-
PYCH_RS07490 (PYCH_15300)	1340158..1340424	+	267	WP_013906252.1	hypothetical protein	-
PYCH_RS09860	1340507..1340728	+	222	WP_148236219.1	hypothetical protein	-
PYCH_RS07500 (PYCH_15320)	1340982..1341608	+	627	WP_013906254.1	hypothetical protein	Antitoxin
PYCH_RS07505 (PYCH_15330)	1341598..1342359	+	762	WP_013906255.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
PYCH_RS10195 (PYCH_15340)	1342373..1342615	+	243	WP_013906256.1	hypothetical protein	-
PYCH_RS07515 (PYCH_15350)	1343156..1344100	+	945	WP_013906257.1	SPFH domain-containing protein	-
PYCH_RS07520 (PYCH_15360)	1344119..1344547	+	429	WP_013906258.1	NfeD family protein	-
PYCH_RS07525 (PYCH_15370)	1344513..1345190	-	678	WP_013906259.1	hypothetical protein	-
PYCH_RS07530 (PYCH_15380)	1345202..1346125	-	924	WP_013906260.1	sodium:calcium antiporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1322704..1342615	19911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (48-250)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 30279.03 Da
• Isoelectric Point: 9.5214

>T10180 WP_013906255.1 NC_015680:1341598-1342359 [Pyrococcus yayanosii CH1]
MLNNELLFKIRESERKGFANFVSKKVGIKNVELVEWDYIIHTILKELEKDPYFRENYVFKGGTCLVKCHLGYYRFSRDLD
FAYRNSEELQEMSRSKLKKFLSEETGKIAETLNCIARDLGLEFYYRDHSDFNNHRYFSFLIGPGWFREIIAYSPAGEKIK
LEFNYAEKFAFKPKTAKAHTLLSWKKVELGVDDYEKYIEFLGNYYPIRVSAYQDKEILIEKVRAILTRREFKLRDLYDLY
KLHQVKGLKIKKI

Nucleotide

• Length: 762 bp

>T10180 NC_015680:1341598-1342359 [Pyrococcus yayanosii CH1]
ATGTTGAATAACGAACTTCTCTTCAAAATTAGGGAGAGCGAGAGGAAGGGATTTGCCAATTTTGTTTCCAAGAAGGTTGG
TATTAAAAACGTAGAGTTGGTTGAGTGGGACTACATCATTCACACCATCCTCAAAGAGCTTGAAAAAGACCCTTATTTTA
GAGAGAACTATGTTTTCAAAGGTGGGACATGCTTGGTTAAGTGCCATCTGGGCTATTACCGCTTTAGCAGGGATTTAGAT
TTTGCCTACAGAAACAGTGAAGAACTCCAAGAGATGTCAAGGAGTAAACTCAAGAAATTCCTAAGTGAAGAGACGGGAAA
AATTGCCGAAACACTGAATTGCATTGCAAGAGACTTGGGATTGGAGTTTTATTACAGAGATCACAGTGACTTTAACAATC
ACCGCTACTTTAGCTTTTTGATAGGGCCCGGCTGGTTTAGAGAAATAATTGCCTATTCTCCCGCAGGAGAGAAGATAAAA
CTGGAGTTCAACTATGCTGAAAAGTTTGCATTTAAACCCAAAACAGCAAAAGCTCACACTCTTCTATCATGGAAAAAGGT
GGAATTGGGAGTTGATGATTATGAGAAATACATTGAGTTTCTCGGGAATTATTATCCCATTAGGGTTTCAGCATATCAGG
ATAAGGAAATTTTGATTGAGAAAGTGAGGGCTATTCTAACAAGGAGGGAATTCAAACTGCGTGATTTGTATGACCTTTAC
AAGCTCCATCAGGTTAAGGGGCTGAAAATTAAAAAAATATGA

Antitoxin

Protein

• Length: 209 a.a.
• Molecular weight: 24318.65 Da
• Isoelectric Point: 9.9271

>AT10180 WP_013906254.1 NC_015680:1340982-1341608 [Pyrococcus yayanosii CH1]
MRPITQILLAEFGGKVITKEDLEKLSTYLKVDDVDYLVNYLIQYGYIIRILRGLYYVKTPVEFSFKSSPTIYKLLSLGMN
KVTKNWYFGLFTALVLNGLTHEHYTTIFIINDKIARTKIIRVNGTPVRIIKTKRKLFDFGIVEKGDMKISDLEKILLDFL
YFGNYGTISKELALRIWREYKDSADWKKLKKYLEKYPESIAKVVGDVE

Nucleotide

• Length: 627 bp

>AT10180 NC_015680:1340982-1341608 [Pyrococcus yayanosii CH1]
ATGAGACCAATTACTCAAATACTCCTGGCTGAATTTGGGGGCAAAGTCATTACAAAAGAAGATCTGGAAAAGCTTTCCAC
TTATTTGAAAGTAGATGATGTAGATTACCTTGTAAACTACCTCATTCAGTATGGCTACATCATAAGAATTCTAAGGGGGC
TCTATTATGTTAAAACGCCAGTTGAGTTCTCTTTTAAGAGCTCTCCCACAATTTACAAACTCCTTTCATTGGGAATGAAC
AAAGTAACGAAAAACTGGTACTTTGGCCTCTTCACAGCATTGGTTCTAAATGGATTAACTCACGAGCACTACACTACAAT
TTTCATAATCAACGACAAGATTGCAAGGACAAAAATCATCCGCGTAAATGGAACTCCGGTCAGAATAATCAAAACCAAAA
GAAAGCTATTTGACTTTGGAATAGTGGAAAAAGGCGATATGAAAATTTCTGATTTAGAAAAAATACTTTTAGATTTCCTC
TACTTTGGAAACTATGGGACAATTTCAAAAGAACTGGCTTTAAGAATCTGGAGAGAATACAAAGATAGTGCAGACTGGAA
GAAGTTGAAGAAGTATTTGGAGAAATATCCAGAGTCCATTGCAAAGGTGGTTGGTGATGTTGAATAA

Structures

Toxin

• AlphaFold DB: F8AGR3

Antitoxin

• AlphaFold DB: F8AGR2

References

(1) Zhen Li et al. (2018) Identification of a functional toxin-antitoxin system located in the genomic island PYG1 of piezophilic hyperthermophilic archaeon Pyrococcus yayanosii. Extremophiles : Life Under Extreme Conditions 22(3):347-357. [PubMed:29335804]


 experimental literature