Detailed information of TA system TA101786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 44161..44720
• Replicon: plasmid pTA143
• Accession: NZ_CP028608
• Organism: Escherichia coli strain 143

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: C9E67_RS28965
• Protein ID: WP_000421248.1
• Coordinates: 44161..44436 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: C9E67_RS28970
• Protein ID: WP_001178089.1
• Coordinates: 44436..44720 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E67_RS28930	39514..39903	-	390	WP_001172748.1	cytochrome b562 family protein	-
C9E67_RS28935	39947..42157	-	2211	WP_000592771.1	catalase/peroxidase KatP	-
C9E67_RS28940	42334..42519	+	186	WP_001302179.1	hypothetical protein	-
C9E67_RS28945	42519..42935	+	417	Protein_35	IS91 family transposase	-
C9E67_RS28950	42935..43501	+	567	Protein_36	IS3-like element IS600 family transposase	-
C9E67_RS28955	43545..43760	+	216	Protein_37	transposase	-
C9E67_RS28960	43860..44066	-	207	WP_001105064.1	hypothetical protein	-
C9E67_RS28965	44161..44436	-	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E67_RS28970	44436..44720	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
C9E67_RS28985	45632..46489	-	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
C9E67_RS28990	46482..46556	-	75	WP_001370046.1	RepA leader peptide Tap	-
C9E67_RS29000	46792..47046	-	255	WP_000083831.1	replication regulatory protein RepA	-
C9E67_RS29005	47286..47624	-	339	WP_000766796.1	hypothetical protein	-
C9E67_RS29010	47662..47871	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
C9E67_RS29015	47917..48378	-	462	WP_001233853.1	thermonuclease family protein	-
C9E67_RS29020	48623..48835	-	213	WP_001302189.1	ANR family transcriptional regulator	-
C9E67_RS29025	48968..49528	-	561	WP_000840472.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / toxB	1..92745	92745

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T101786 WP_000421248.1 NZ_CP028608:c44436-44161 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T101786 NZ_CP028608:c44436-44161 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT101786 WP_001178089.1 NZ_CP028608:c44720-44436 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT101786 NZ_CP028608:c44720-44436 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8