Detailed information of TA system TA101776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3648755..3649233
• Replicon: chromosome
• Accession: NZ_CP028607
• Organism: Escherichia coli strain 143

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: C9E67_RS19550
• Protein ID: WP_001303876.1
• Coordinates: 3648946..3649233 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: C9E67_RS19545
• Protein ID: WP_000536233.1
• Coordinates: 3648755..3648946 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E67_RS19510	3644159..3644929	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
C9E67_RS19515	3644951..3645697	-	747	WP_000788751.1	ATP-binding protein	-
C9E67_RS19520	3645704..3646795	-	1092	WP_001205823.1	hypothetical protein	-
C9E67_RS19525	3646874..3647329	+	456	WP_000273724.1	hypothetical protein	-
C9E67_RS19530	3647536..3647961	-	426	WP_000693855.1	Rha family transcriptional regulator	-
C9E67_RS19535	3647945..3648217	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
C9E67_RS19540	3648326..3648727	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
C9E67_RS19545	3648755..3648946	+	192	WP_000536233.1	hypothetical protein	Antitoxin
C9E67_RS19550	3648946..3649233	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C9E67_RS19560	3649511..3649666	+	156	WP_000379575.1	DUF1391 family protein	-
C9E67_RS19565	3649808..3650197	+	390	WP_000394511.1	hypothetical protein	-
C9E67_RS19570	3650384..3650569	-	186	WP_001133046.1	hypothetical protein	-
C9E67_RS19580	3651143..3651331	+	189	WP_000413705.1	cell division inhibition protein DicB	-
C9E67_RS19585	3651328..3651519	+	192	WP_001098307.1	DUF1482 family protein	-
C9E67_RS19590	3651613..3654084	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T101776 WP_001303876.1 NZ_CP028607:3648946-3649233 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T101776 NZ_CP028607:3648946-3649233 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT101776 WP_000536233.1 NZ_CP028607:3648755-3648946 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT101776 NZ_CP028607:3648755-3648946 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA