Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51328..51853 Replicon plasmid pTA144
Accession NZ_CP028606
Organism Escherichia coli strain 144

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag C9E68_RS28595 Protein ID WP_001159868.1
Coordinates 51548..51853 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C9E68_RS28590 Protein ID WP_000813634.1
Coordinates 51328..51546 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E68_RS28555 46611..46841 + 231 WP_010891288.1 hypothetical protein -
C9E68_RS28560 46962..47702 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
C9E68_RS28565 47987..48964 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
C9E68_RS28575 49372..49572 - 201 WP_000708307.1 hypothetical protein -
C9E68_RS28580 49569..50189 - 621 WP_001248529.1 hypothetical protein -
C9E68_RS28585 50186..50869 - 684 WP_010891291.1 DNA-binding protein -
C9E68_RS28590 51328..51546 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C9E68_RS28595 51548..51853 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
C9E68_RS28600 51854..52660 + 807 WP_000016989.1 site-specific integrase -
C9E68_RS28610 53383..54594 - 1212 Protein_52 IS3 family transposase -
C9E68_RS28615 54670..55425 + 756 WP_000852148.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..95648 95648


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T101756 WP_001159868.1 NZ_CP028606:51548-51853 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T101756 NZ_CP028606:51548-51853 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT101756 WP_000813634.1 NZ_CP028606:51328-51546 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT101756 NZ_CP028606:51328-51546 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References