Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 33567..34211 Replicon plasmid pTA144-2
Accession NZ_CP028605
Organism Escherichia coli strain 144

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag C9E68_RS28265 Protein ID WP_000833471.1
Coordinates 33567..33749 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag C9E68_RS28270 Protein ID WP_000466317.1
Coordinates 33774..34211 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E68_RS28225 30566..30889 + 324 WP_000432877.1 hypothetical protein -
C9E68_RS28230 30930..31154 + 225 WP_000866040.1 hypothetical protein -
C9E68_RS28235 31250..31474 + 225 WP_000749828.1 hypothetical protein -
C9E68_RS28240 31525..31791 + 267 WP_000702249.1 hypothetical protein -
C9E68_RS28245 31781..32023 + 243 WP_001450803.1 hypothetical protein -
C9E68_RS28255 32463..32792 + 330 WP_000449468.1 hypothetical protein -
C9E68_RS28260 32850..33128 + 279 WP_000545936.1 hypothetical protein -
C9E68_RS28265 33567..33749 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
C9E68_RS28270 33774..34211 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C9E68_RS28275 34240..34944 - 705 WP_001279865.1 hypothetical protein -
C9E68_RS28280 34941..35702 - 762 WP_001097937.1 conjugal transfer protein TraL -
C9E68_RS28285 35712..36119 - 408 WP_001256557.1 hypothetical protein -
C9E68_RS28290 36465..36803 + 339 WP_000753495.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..40350 40350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T101754 WP_000833471.1 NZ_CP028605:33567-33749 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T101754 NZ_CP028605:33567-33749 [Escherichia coli]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT101754 WP_000466317.1 NZ_CP028605:33774-34211 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT101754 NZ_CP028605:33774-34211 [Escherichia coli]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGCTCTTATTCTGTTGACGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTTAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References