Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 31525..32023 Replicon plasmid pTA144-2
Accession NZ_CP028605
Organism Escherichia coli strain 144

Toxin (Protein)


Gene name TsxA Uniprot ID A0A0K6A0X9
Locus tag C9E68_RS28240 Protein ID WP_000702249.1
Coordinates 31525..31791 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A3H7VMJ5
Locus tag C9E68_RS28245 Protein ID WP_001450803.1
Coordinates 31781..32023 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E68_RS28195 26642..26929 - 288 WP_000332249.1 helix-turn-helix transcriptional regulator -
C9E68_RS28200 27128..27466 + 339 WP_000612441.1 helix-turn-helix domain-containing protein -
C9E68_RS28205 27884..28888 - 1005 WP_001208090.1 replication initiation protein -
C9E68_RS28225 30566..30889 + 324 WP_000432877.1 hypothetical protein -
C9E68_RS28230 30930..31154 + 225 WP_000866040.1 hypothetical protein -
C9E68_RS28235 31250..31474 + 225 WP_000749828.1 hypothetical protein -
C9E68_RS28240 31525..31791 + 267 WP_000702249.1 hypothetical protein Toxin
C9E68_RS28245 31781..32023 + 243 WP_001450803.1 hypothetical protein Antitoxin
C9E68_RS28255 32463..32792 + 330 WP_000449468.1 hypothetical protein -
C9E68_RS28260 32850..33128 + 279 WP_000545936.1 hypothetical protein -
C9E68_RS28265 33567..33749 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin -
C9E68_RS28270 33774..34211 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin -
C9E68_RS28275 34240..34944 - 705 WP_001279865.1 hypothetical protein -
C9E68_RS28280 34941..35702 - 762 WP_001097937.1 conjugal transfer protein TraL -
C9E68_RS28285 35712..36119 - 408 WP_001256557.1 hypothetical protein -
C9E68_RS28290 36465..36803 + 339 WP_000753495.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..40350 40350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10242.76 Da        Isoelectric Point: 9.9280

>T101753 WP_000702249.1 NZ_CP028605:31525-31791 [Escherichia coli]
MKIRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Download         Length: 267 bp

>T101753 NZ_CP028605:31525-31791 [Escherichia coli]
ATGAAGATACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTAATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9288.69 Da        Isoelectric Point: 9.7120

>AT101753 WP_001450803.1 NZ_CP028605:31781-32023 [Escherichia coli]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Download         Length: 243 bp

>AT101753 NZ_CP028605:31781-32023 [Escherichia coli]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K6A0X9

Antitoxin

Source ID Structure
AlphaFold DB A0A3H7VMJ5

References