Detailed information of TA system TA101716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 7354..7847
• Replicon: plasmid pTA147-2
• Accession: NZ_CP028601
• Organism: Escherichia coli strain 147

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9Y9W8
• Locus tag: C9E69_RS27550
• Protein ID: WP_001230709.1
• Coordinates: 7354..7608 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: U9Y4R8
• Locus tag: C9E69_RS27555
• Protein ID: WP_000151456.1
• Coordinates: 7605..7847 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E69_RS28675	2572..2733	+	162	WP_001128311.1	hypothetical protein	-
C9E69_RS27510	2927..3088	+	162	WP_001434039.1	type I toxin-antitoxin system Hok family toxin	-
C9E69_RS27515	3238..3834	+	597	WP_000125164.1	hypothetical protein	-
C9E69_RS28870	3852..4136	-	285	WP_001162371.1	hypothetical protein	-
C9E69_RS27525	4210..4875	-	666	WP_000532148.1	P-loop NTPase	-
C9E69_RS27535	5463..5792	+	330	WP_001542022.1	hypothetical protein	-
C9E69_RS27540	6196..6711	+	516	WP_001025393.1	J domain-containing protein	-
C9E69_RS27545	6815..7357	+	543	WP_001083904.1	hypothetical protein	-
C9E69_RS27550	7354..7608	+	255	WP_001230709.1	hypothetical protein	Toxin
C9E69_RS27555	7605..7847	+	243	WP_000151456.1	hypothetical protein	Antitoxin
C9E69_RS27560	8053..8302	+	250	Protein_12	hypothetical protein	-
C9E69_RS27565	8418..8705	+	288	WP_000673430.1	hypothetical protein	-
C9E69_RS27570	8715..9131	+	417	WP_001708403.1	hypothetical protein	-
C9E69_RS27575	9128..9439	+	312	WP_001310436.1	hypothetical protein	-
C9E69_RS27580	9849..10031	-	183	WP_001401700.1	hypothetical protein	-
C9E69_RS27585	10589..10771	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
C9E69_RS27590	10796..11233	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
C9E69_RS27595	11364..11684	-	321	WP_000206710.1	hypothetical protein	-
C9E69_RS27600	11695..11958	-	264	WP_000224227.1	hypothetical protein	-
C9E69_RS27605	11960..12451	-	492	WP_001167293.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..59888	59888

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Antitoxin

• Toxin YoeB (5-84)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9942.47 Da
• Isoelectric Point: 10.1206

>T101716 WP_001230709.1 NZ_CP028601:7354-7608 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Nucleotide

• Length: 255 bp

>T101716 NZ_CP028601:7354-7608 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9136.45 Da
• Isoelectric Point: 7.2775

>AT101716 WP_000151456.1 NZ_CP028601:7605-7847 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Nucleotide

• Length: 243 bp

>AT101716 NZ_CP028601:7605-7847 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A3G9H8F0

Antitoxin

• AlphaFold DB: A0A3G9GNF5