Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2561378..2561849 Replicon chromosome
Accession NZ_CP028600
Organism Escherichia coli strain 147

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C9E69_RS13160 Protein ID WP_001303511.1
Coordinates 2561378..2561656 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C9E69_RS13165 Protein ID WP_001302048.1
Coordinates 2561658..2561849 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E69_RS13130 2558313..2559398 - 1086 Protein_2516 exonuclease -
C9E69_RS13135 2559491..2559682 - 192 WP_001090200.1 DUF1482 family protein -
C9E69_RS13140 2559679..2559867 - 189 WP_000449192.1 cell division inhibition protein DicB -
C9E69_RS13150 2560436..2560654 - 219 WP_001171930.1 hypothetical protein -
C9E69_RS13155 2560726..2561025 - 300 WP_001240334.1 hypothetical protein -
C9E69_RS13160 2561378..2561656 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C9E69_RS13165 2561658..2561849 - 192 WP_001302048.1 hypothetical protein Antitoxin
C9E69_RS13170 2561870..2562241 - 372 WP_001169686.1 hypothetical protein -
C9E69_RS13175 2562339..2562641 + 303 WP_000172738.1 transcriptional regulator -
C9E69_RS13180 2562638..2563063 + 426 WP_000693943.1 Rha family transcriptional regulator -
C9E69_RS13185 2563086..2564048 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
C9E69_RS13190 2564055..2564795 + 741 WP_000788938.1 ATP-binding protein -
C9E69_RS13195 2564821..2565590 + 770 Protein_2528 DUF1627 domain-containing protein -
C9E69_RS13200 2565606..2566001 + 396 WP_001118159.1 DUF977 family protein -
C9E69_RS13205 2566058..2566642 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 2545185..2644247 99062


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T101699 WP_001303511.1 NZ_CP028600:c2561656-2561378 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T101699 NZ_CP028600:c2561656-2561378 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT101699 WP_001302048.1 NZ_CP028600:c2561849-2561658 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT101699 NZ_CP028600:c2561849-2561658 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References