Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 33551..34195 Replicon plasmid pTA150-2
Accession NZ_CP028594
Organism Escherichia coli strain 150

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag C9E72_RS28720 Protein ID WP_000833471.1
Coordinates 33551..33733 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag C9E72_RS28725 Protein ID WP_000466317.1
Coordinates 33758..34195 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E72_RS28675 28802..29026 + 225 WP_000749828.1 hypothetical protein -
C9E72_RS28680 29077..29343 + 267 WP_000702249.1 hypothetical protein -
C9E72_RS28685 29333..29575 + 243 WP_001450803.1 hypothetical protein -
C9E72_RS28695 30015..30344 + 330 WP_000449468.1 hypothetical protein -
C9E72_RS28700 30454..30834 + 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
C9E72_RS28705 30831..31190 + 360 WP_137049499.1 IS66 family insertion sequence element accessory protein TnpB -
C9E72_RS28710 31211..32748 + 1538 Protein_36 IS66 family transposase -
C9E72_RS28715 32834..33112 + 279 WP_000545936.1 hypothetical protein -
C9E72_RS28720 33551..33733 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
C9E72_RS28725 33758..34195 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C9E72_RS28730 34224..34928 - 705 WP_001279865.1 hypothetical protein -
C9E72_RS28735 34925..35686 - 762 WP_001097937.1 conjugal transfer protein TraL -
C9E72_RS28740 35696..36103 - 408 WP_001256557.1 hypothetical protein -
C9E72_RS28745 36449..36787 + 339 WP_000753495.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..40334 40334


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T101609 WP_000833471.1 NZ_CP028594:33551-33733 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T101609 NZ_CP028594:33551-33733 [Escherichia coli]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT101609 WP_000466317.1 NZ_CP028594:33758-34195 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT101609 NZ_CP028594:33758-34195 [Escherichia coli]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGCTCTTATTCTGTTGACGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTTAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References