101608

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 29077..29575 Replicon plasmid pTA150-2
Accession NZ_CP028594
Organism Escherichia coli strain 150

Toxin (Protein)


Gene name TsxA Uniprot ID A0A0K6A0X9
Locus tag C9E72_RS28680 Protein ID WP_000702249.1
Coordinates 29077..29343 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A3H7VMJ5
Locus tag C9E72_RS28685 Protein ID WP_001450803.1
Coordinates 29333..29575 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9E72_RS28635 24194..24481 - 288 WP_000332249.1 helix-turn-helix transcriptional regulator -
C9E72_RS28640 24680..25018 + 339 WP_000612441.1 helix-turn-helix domain-containing protein -
C9E72_RS28645 25436..26440 - 1005 WP_001208090.1 replication initiation protein -
C9E72_RS28665 28118..28441 + 324 WP_000432877.1 hypothetical protein -
C9E72_RS28670 28482..28706 + 225 WP_000866040.1 hypothetical protein -
C9E72_RS28675 28802..29026 + 225 WP_000749828.1 hypothetical protein -
C9E72_RS28680 29077..29343 + 267 WP_000702249.1 hypothetical protein Toxin
C9E72_RS28685 29333..29575 + 243 WP_001450803.1 hypothetical protein Antitoxin
C9E72_RS28695 30015..30344 + 330 WP_000449468.1 hypothetical protein -
C9E72_RS28700 30454..30834 + 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
C9E72_RS28705 30831..31190 + 360 WP_137049499.1 IS66 family insertion sequence element accessory protein TnpB -
C9E72_RS28710 31211..32748 + 1538 Protein_36 IS66 family transposase -
C9E72_RS28715 32834..33112 + 279 WP_000545936.1 hypothetical protein -
C9E72_RS28720 33551..33733 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin -
C9E72_RS28725 33758..34195 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..40334 40334


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(10-110)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10242.76 Da        Isoelectric Point: 9.9280

>T101608 WP_000702249.1 NZ_CP028594:29077-29343 [Escherichia coli]
MKIRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Download         Length: 267 bp

>T101608 NZ_CP028594:29077-29343 [Escherichia coli]
ATGAAGATACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTAATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9288.69 Da        Isoelectric Point: 9.7120

>AT101608 WP_001450803.1 NZ_CP028594:29333-29575 [Escherichia coli]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Download         Length: 243 bp

>AT101608 NZ_CP028594:29333-29575 [Escherichia coli]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K6A0X9

Antitoxin

Source ID Structure
AlphaFold DB A0A3H7VMJ5

References