Detailed information of TA system TA101606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5001717..5002312
• Replicon: chromosome
• Accession: NZ_CP028592
• Organism: Escherichia coli strain 150

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: C9E72_RS25770
• Protein ID: WP_000239579.1
• Coordinates: 5001717..5002067 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: C9E72_RS25775
• Protein ID: WP_001223210.1
• Coordinates: 5002061..5002312 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E72_RS25750	4997163..4998185	-	1023	WP_001301928.1	ABC transporter permease	-
C9E72_RS25755	4998199..4999701	-	1503	WP_000205805.1	sugar ABC transporter ATP-binding protein	-
C9E72_RS25760	4999841..5000797	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
C9E72_RS25765	5001107..5001637	+	531	WP_000055075.1	inorganic diphosphatase	-
C9E72_RS25770	5001717..5002067	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
C9E72_RS25775	5002061..5002312	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
C9E72_RS25780	5002524..5002865	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
C9E72_RS25785	5002868..5006647	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T101606 WP_000239579.1 NZ_CP028592:c5002067-5001717 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T101606 NZ_CP028592:c5002067-5001717 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT101606 WP_001223210.1 NZ_CP028592:c5002312-5002061 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT101606 NZ_CP028592:c5002312-5002061 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8