Detailed information of TA system TA101570

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4602565..4603400
• Replicon: chromosome
• Accession: NZ_CP028589
• Organism: Escherichia coli strain WCHEC4533

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J1YPH7
• Locus tag: DBR03_RS24585
• Protein ID: WP_029701676.1
• Coordinates: 4603023..4603400 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: DBR03_RS24580
• Protein ID: WP_001295723.1
• Coordinates: 4602565..4602933 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR03_RS24545	4598227..4598907	+	681	WP_029702103.1	WYL domain-containing protein	-
DBR03_RS24550	4599055..4599732	+	678	WP_001097302.1	hypothetical protein	-
DBR03_RS24555	4599738..4599971	+	234	WP_001278293.1	DUF905 family protein	-
DBR03_RS24560	4600061..4600879	+	819	WP_001175165.1	DUF945 domain-containing protein	-
DBR03_RS24565	4601145..4601624	+	480	WP_001564060.1	antirestriction protein	-
DBR03_RS24570	4601640..4602116	+	477	WP_021553055.1	RadC family protein	-
DBR03_RS24575	4602181..4602402	+	222	WP_000692345.1	DUF987 domain-containing protein	-
DBR03_RS24580	4602565..4602933	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DBR03_RS24585	4603023..4603400	+	378	WP_029701676.1	TA system toxin CbtA family protein	Toxin
DBR03_RS27125	4603397..4603819	+	423	WP_001564064.1	DUF957 domain-containing protein	-
DBR03_RS24600	4603904..4604746	+	843	WP_022645115.1	DUF4942 domain-containing protein	-
DBR03_RS24605	4605495..4607033	+	1539	WP_001187196.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14066.04 Da
• Isoelectric Point: 7.8045

>T101570 WP_029701676.1 NZ_CP028589:4603023-4603400 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T101570 NZ_CP028589:4603023-4603400 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT101570 WP_001295723.1 NZ_CP028589:4602565-4602933 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT101570 NZ_CP028589:4602565-4602933 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J1YPH7

Antitoxin

• AlphaFold DB: S1NM52