Detailed information of TA system TA101566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4168218..4168705
• Replicon: chromosome
• Accession: NZ_CP028589
• Organism: Escherichia coli strain WCHEC4533

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: DBR03_RS22265
• Protein ID: WP_001520461.1
• Coordinates: 4168218..4168469 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: DBR03_RS22270
• Protein ID: WP_001520458.1
• Coordinates: 4168472..4168705 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR03_RS22240	4163830..4165116	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
DBR03_RS22245	4165116..4166105	+	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
DBR03_RS22250	4166102..4166923	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
DBR03_RS22255	4166926..4167303	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
DBR03_RS22260	4167310..4168158	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
DBR03_RS22265	4168218..4168469	-	252	WP_001520461.1	CcdB family protein	Toxin
DBR03_RS22270	4168472..4168705	-	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
DBR03_RS22275	4168791..4169270	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
DBR03_RS22280	4169461..4171323	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
DBR03_RS22285	4171316..4171846	-	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
DBR03_RS22290	4171954..4173285	-	1332	WP_001520455.1	MFS transporter	-
DBR03_RS22295	4173344..4173631	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T101566 WP_001520461.1 NZ_CP028589:c4168469-4168218 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T101566 NZ_CP028589:c4168469-4168218 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT101566 WP_001520458.1 NZ_CP028589:c4168705-4168472 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT101566 NZ_CP028589:c4168705-4168472 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4