Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3723537..3724155 Replicon chromosome
Accession NZ_CP028589
Organism Escherichia coli strain WCHEC4533

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DBR03_RS20190 Protein ID WP_001291435.1
Coordinates 3723937..3724155 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DBR03_RS20185 Protein ID WP_000344800.1
Coordinates 3723537..3723911 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR03_RS20175 3718626..3719819 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DBR03_RS20180 3719842..3722991 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
DBR03_RS20185 3723537..3723911 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DBR03_RS20190 3723937..3724155 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DBR03_RS20195 3724327..3724878 + 552 WP_001522034.1 maltose O-acetyltransferase -
DBR03_RS20200 3724994..3725464 + 471 WP_001490888.1 YlaC family protein -
DBR03_RS20205 3725628..3727178 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DBR03_RS20210 3727220..3727573 - 354 WP_000878140.1 DUF1428 domain-containing protein -
DBR03_RS20220 3727952..3728263 + 312 WP_000409911.1 MGMT family protein -
DBR03_RS20225 3728294..3728866 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T101563 WP_001291435.1 NZ_CP028589:3723937-3724155 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T101563 NZ_CP028589:3723937-3724155 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT101563 WP_000344800.1 NZ_CP028589:3723537-3723911 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT101563 NZ_CP028589:3723537-3723911 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References