Detailed information of TA system TA101558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2436363..2436889
• Replicon: chromosome
• Accession: NZ_CP028589
• Organism: Escherichia coli strain WCHEC4533

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: DBR03_RS13880
• Protein ID: WP_000323025.1
• Coordinates: 2436602..2436889 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: DBR03_RS13875
• Protein ID: WP_000534858.1
• Coordinates: 2436363..2436602 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR03_RS13850	2431625..2432239	+	615	WP_001265627.1	hypothetical protein	-
DBR03_RS13855	2432242..2433264	+	1023	WP_000915483.1	DUF4868 domain-containing protein	-
DBR03_RS13860	2433748..2435061	+	1314	WP_000589005.1	ISNCY family transposase	-
DBR03_RS13865	2435498..2435830	-	333	WP_001301033.1	protein FlxA	-
DBR03_RS13870	2436033..2436338	-	306	WP_001326990.1	hypothetical protein	-
DBR03_RS13875	2436363..2436602	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
DBR03_RS13880	2436602..2436889	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
DBR03_RS13885	2436961..2437116	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
DBR03_RS13890	2437333..2437584	+	252	WP_000980994.1	hypothetical protein	-
DBR03_RS13895	2437651..2437929	+	279	WP_012304870.1	hypothetical protein	-
DBR03_RS13900	2437931..2438980	+	1050	WP_001265199.1	DUF968 domain-containing protein	-
DBR03_RS13905	2438994..2439746	+	753	WP_001047135.1	antitermination protein	-
DBR03_RS13910	2440024..2440113	-	90	WP_120795389.1	hypothetical protein	-
DBR03_RS13915	2440168..2440380	-	213	WP_000087756.1	cold shock-like protein CspF	-
DBR03_RS13920	2440681..2440896	+	216	WP_000066484.1	cold shock-like protein CspB	-
DBR03_RS13925	2441347..2441805	+	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2407513..2479634	72121
	-	flank	IS/Tn	-	-	2441347..2441805	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T101558 WP_000323025.1 NZ_CP028589:2436602-2436889 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T101558 NZ_CP028589:2436602-2436889 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT101558 WP_000534858.1 NZ_CP028589:2436363-2436602 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT101558 NZ_CP028589:2436363-2436602 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8