Detailed information of TA system TA101546

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 968662..969322
• Replicon: chromosome
• Accession: NZ_CP028589
• Organism: Escherichia coli strain WCHEC4533

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: DBR03_RS06685
• Protein ID: WP_029701967.1
• Coordinates: 968909..969322 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: S1PPB1
• Locus tag: DBR03_RS06680
• Protein ID: WP_000354046.1
• Coordinates: 968662..968928 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR03_RS06655	963950..964693	+	744	WP_000951964.1	SDR family oxidoreductase	-
DBR03_RS06660	964750..966183	-	1434	WP_001336277.1	6-phospho-beta-glucosidase BglA	-
DBR03_RS06665	966228..966539	+	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
DBR03_RS06670	966703..967362	+	660	WP_000250274.1	hemolysin III family protein	-
DBR03_RS06675	967439..968419	-	981	WP_000886083.1	tRNA-modifying protein YgfZ	-
DBR03_RS06680	968662..968928	+	267	WP_000354046.1	FAD assembly factor SdhE	Antitoxin
DBR03_RS06685	968909..969322	+	414	WP_029701967.1	protein YgfX	Toxin
DBR03_RS06690	969356..969877	-	522	WP_001055874.1	flavodoxin FldB	-
DBR03_RS06695	969989..970885	+	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
DBR03_RS06700	970909..971619	+	711	WP_029701969.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
DBR03_RS06705	971625..973358	+	1734	WP_029701971.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16305.25 Da
• Isoelectric Point: 11.7064

>T101546 WP_029701967.1 NZ_CP028589:968909-969322 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLLHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Nucleotide

• Length: 414 bp

>T101546 NZ_CP028589:968909-969322 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGCTTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATCAAGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT101546 WP_000354046.1 NZ_CP028589:968662-968928 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT101546 NZ_CP028589:968662-968928 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTCAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAATTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QD57