101532

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 61071..61672 Replicon plasmid p1_000533
Accession NZ_CP028585
Organism Escherichia coli strain WCHEC4533

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag DBR03_RS00355 Protein ID WP_001216041.1
Coordinates 61071..61451 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag DBR03_RS00360 Protein ID WP_001190712.1
Coordinates 61451..61672 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR03_RS00320 56089..57117 + 1029 WP_087503522.1 tyrosine-type recombinase/integrase -
DBR03_RS00325 57192..57536 + 345 WP_087503523.1 hypothetical protein -
DBR03_RS00330 57533..58009 + 477 WP_087503524.1 hypothetical protein -
DBR03_RS00335 58006..58500 + 495 WP_000640903.1 dUTP diphosphatase -
DBR03_RS00340 58515..59177 + 663 WP_060877112.1 DUF2829 domain-containing protein -
DBR03_RS00345 59184..59951 + 768 WP_087503525.1 hypothetical protein -
DBR03_RS00350 59941..61035 + 1095 WP_087503526.1 hypothetical protein -
DBR03_RS00355 61071..61451 - 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
DBR03_RS00360 61451..61672 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DBR03_RS00365 61745..62134 - 390 WP_032153799.1 DNA repair protein -
DBR03_RS00370 62258..62509 - 252 WP_032153798.1 DNA polymerase III subunit theta -
DBR03_RS00375 62512..62712 - 201 WP_001408980.1 hypothetical protein -
DBR03_RS00380 62824..63594 - 771 WP_087503527.1 hypothetical protein -
DBR03_RS00385 63591..63899 - 309 WP_087503528.1 hypothetical protein -
DBR03_RS00390 63899..64192 - 294 WP_087503529.1 hypothetical protein -
DBR03_RS00395 64205..64621 - 417 WP_063074823.1 hypothetical protein -
DBR03_RS00400 64907..65167 - 261 WP_087503530.1 eaa protein -
DBR03_RS00405 65164..65853 - 690 WP_087503531.1 DUF551 domain-containing protein -
DBR03_RS00410 65850..66059 - 210 WP_000951710.1 hypothetical protein -
DBR03_RS00415 66061..66249 - 189 WP_000797279.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..92537 92537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T101532 WP_001216041.1 NZ_CP028585:c61451-61071 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T101532 NZ_CP028585:c61451-61071 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCCGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAATTATTTGGTTCCAATATTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT101532 WP_001190712.1 NZ_CP028585:c61672-61451 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT101532 NZ_CP028585:c61672-61451 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGAGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References