Detailed information of TA system TA101523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5366894..5367525
• Replicon: chromosome
• Accession: NZ_CP028583
• Organism: Klebsiella pneumoniae strain WCHKP36

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: CEA75_RS28630
• Protein ID: WP_002882817.1
• Coordinates: 5366894..5367277 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CEA75_RS28635
• Protein ID: WP_002882814.1
• Coordinates: 5367277..5367525 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA75_RS28615	5364260..5365162	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
CEA75_RS28620	5365159..5365794	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
CEA75_RS28625	5365791..5366720	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
CEA75_RS28630	5366894..5367277	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
CEA75_RS28635	5367277..5367525	-	249	WP_002882814.1	ribbon-helix-helix domain-containing protein	Antitoxin
CEA75_RS28640	5367724..5368641	+	918	WP_002882812.1	alpha/beta hydrolase	-
CEA75_RS28645	5368655..5369644	-	990	WP_002882811.1	fatty acid biosynthesis protein FabY	-
CEA75_RS28650	5369641..5370078	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CEA75_RS28655	5370075..5370935	-	861	WP_002882807.1	virulence factor BrkB family protein	-
CEA75_RS28660	5370929..5371528	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (18-49)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T101523 WP_002882817.1 NZ_CP028583:c5367277-5366894 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T101523 NZ_CP028583:c5367277-5366894 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9786.10 Da
• Isoelectric Point: 4.3509

>AT101523 WP_002882814.1 NZ_CP028583:c5367525-5367277 [Klebsiella pneumoniae]
MSMMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRK
EW

Nucleotide

• Length: 249 bp

>AT101523 NZ_CP028583:c5367525-5367277 [Klebsiella pneumoniae]
ATGAGCATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCT
CAAAGTGCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTA
AAGATACCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAG
GAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8