Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 134950..135593 Replicon plasmid pKPC2_020036
Accession NZ_CP028582
Organism Klebsiella pneumoniae strain WCHKP36

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CEA75_RS01310 Protein ID WP_001044770.1
Coordinates 135177..135593 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CEA75_RS01305 Protein ID WP_001261282.1
Coordinates 134950..135180 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEA75_RS01270 130173..130952 - 780 WP_013214009.1 site-specific integrase -
CEA75_RS01275 131136..132140 - 1005 WP_011977814.1 hypothetical protein -
CEA75_RS01280 132170..132373 - 204 WP_011977813.1 hypothetical protein -
CEA75_RS01285 132419..132940 - 522 WP_013214008.1 hypothetical protein -
CEA75_RS01290 132998..133348 - 351 WP_032738650.1 hypothetical protein -
CEA75_RS01295 133427..134371 - 945 WP_011977810.1 hypothetical protein -
CEA75_RS01300 134532..134966 - 435 WP_015493090.1 hypothetical protein -
CEA75_RS01305 134950..135180 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CEA75_RS01310 135177..135593 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CEA75_RS01315 135667..137229 + 1563 WP_004206609.1 AAA family ATPase -
CEA75_RS01320 137214..138236 + 1023 WP_000361404.1 helicase UvrD -
CEA75_RS01325 138492..139189 + 698 Protein_202 IS1 family transposase -
CEA75_RS01330 139218..139490 + 273 Protein_203 IS5/IS1182 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / catA2 / blaKPC-2 - 1..149258 149258


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T101501 WP_001044770.1 NZ_CP028582:135177-135593 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T101501 NZ_CP028582:135177-135593 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT101501 WP_001261282.1 NZ_CP028582:134950-135180 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT101501 NZ_CP028582:134950-135180 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References