Detailed information of TA system TA101500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1083..1557
• Replicon: plasmid p3_020036
• Accession: NZ_CP028581
• Organism: Klebsiella pneumoniae strain WCHKP36

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: CEA75_RS00280
• Protein ID: WP_004197762.1
• Coordinates: 1083..1349 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: CEA75_RS00285
• Protein ID: WP_000879771.1
• Coordinates: 1333..1557 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA75_RS00270	52..597	+	546	WP_023317533.1	hypothetical protein	-
CEA75_RS00275	663..1013	+	351	WP_004197756.1	hypothetical protein	-
CEA75_RS00280	1083..1349	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEA75_RS00285	1333..1557	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
CEA75_RS00290	2224..2436	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
CEA75_RS00295	2443..2952	-	510	WP_032433972.1	MbeB family mobilization protein	-
CEA75_RS00300	2927..3691	-	765	Protein_7	nuclease	-
CEA75_RS00305	3630..3974	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
CEA75_RS00310	4325..4555	-	231	Protein_9	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T101500 WP_004197762.1 NZ_CP028581:c1349-1083 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T101500 NZ_CP028581:c1349-1083 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT101500 WP_000879771.1 NZ_CP028581:c1557-1333 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT101500 NZ_CP028581:c1557-1333 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5