Detailed information of TA system TA101494

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3681106..3681800
• Replicon: chromosome
• Accession: NZ_CP028578
• Organism: Escherichia coli strain WCHEC005784

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: DBQ99_RS20225
• Protein ID: WP_001094398.1
• Coordinates: 3681106..3681474 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: DBQ99_RS20230
• Protein ID: WP_077249034.1
• Coordinates: 3681495..3681800 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBQ99_RS20205	3676279..3677235	+	957	WP_000643333.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
DBQ99_RS20210	3677232..3679430	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
DBQ99_RS20215	3679440..3680396	+	957	WP_000121359.1	molybdenum cofactor insertion chaperone PaoD	-
DBQ99_RS20220	3680447..3680785	+	339	Protein_3487	LysR family transcriptional regulator	-
DBQ99_RS20225	3681106..3681474	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
DBQ99_RS20230	3681495..3681800	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DBQ99_RS20235	3681904..3682548	-	645	WP_000086755.1	hypothetical protein	-
DBQ99_RS20240	3682567..3682788	-	222	WP_000692345.1	DUF987 domain-containing protein	-
DBQ99_RS20245	3682857..3683333	-	477	WP_001424026.1	RadC family protein	-
DBQ99_RS20250	3683349..3683822	-	474	WP_000855059.1	antirestriction protein	-
DBQ99_RS20260	3684164..3684985	-	822	WP_001234565.1	DUF945 domain-containing protein	-
DBQ99_RS20270	3685086..3685294	-	209	Protein_3495	DUF905 domain-containing protein	-
DBQ99_RS20275	3685395..3685850	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3654617..3697459	42842

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T101494 WP_001094398.1 NZ_CP028578:c3681474-3681106 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T101494 NZ_CP028578:c3681474-3681106 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT101494 WP_077249034.1 NZ_CP028578:c3681800-3681495 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT101494 NZ_CP028578:c3681800-3681495 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4